{"id":54,"date":"2022-03-24T17:55:33","date_gmt":"2022-03-24T14:55:33","guid":{"rendered":"http:\/\/localhost\/hibrigen\/en\/?page_id=54"},"modified":"2022-03-24T19:19:42","modified_gmt":"2022-03-24T16:19:42","slug":"covid-19-rt-pcr-detection-kit","status":"publish","type":"page","link":"https:\/\/hibrigen.com\/en\/covid-19-rt-pcr-detection-kit\/","title":{"rendered":"Covid-19 RT-PCR Detection Kit"},"content":{"rendered":"
\n
\n
\n\t\t\t\t\t\t\t\t\t\"Covid-19\n\t\t\t\t\t\t\t\t<\/div>\n
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\n
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Covid-19 RT-PCR Detection Kit<\/h3>\n<\/p><\/div>\n<\/p><\/div>\n
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MD-COVID19-01<\/h4>\n<\/p>\n

<\/p>\n

Description<\/h5>\n<\/p>\n

\n\t\t\t\t\t\t\t\t\t\t\tThe HibriGen COVID-19 RT-PCR Kit is used for the qualitative detection of the SARS-CoV-2 (2019-nCoV) virus by RNA obtained from human nasopharyngeal aspirate \/ lavage, bronchoalveolar lavage, nasopharyngeal swab, oropharyngeal swab, and sputum samples.\n\t\t\t\t\t\t\t\t\t\t<\/p>\n

\n\t\t\t\t\t\t\t\t\t\t\tThe method of operation of the kit is the single-step RT-PCR (real-time fluorescent PCR) technology, which collects the cDNA mapping (Reverse Transcription) from the RNA of the SARS-CoV-2 (2019-nCoV) virus and the real-time amplification (RT-PCR) of the cDNA in one step.\n\t\t\t\t\t\t\t\t\t\t<\/p>\n

\n\t\t\t\t\t\t\t\t\t\t\tPrimers and probes included in the HibriGen COVID-19 RT-PCR Kit are specific to sequences of the protected RdRp gene (FAM), N gene (Hex) and Human RPII gene for reaction internal control (Cy5) as target sites. It has been designed in accordance with RT-PCR technology from a single step.\n\t\t\t\t\t\t\t\t\t\t<\/p>\n

\n\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t<\/p>\n

\n\t\t\t\t\t\t\t\t\t\t\tKit Contents
\n\t\t\t\t\t\t\t\t\t\t<\/h5>\n

\n\t\t\t\t\t\t\t\t\t\t\t<\/strong>\n\t\t\t\t\t\t\t\t\t\t<\/p>\n

\n\n\n\n\n\n
\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t50 Test<\/p>\n<\/td>\n

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\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t100 Test<\/p>\n<\/td>\n

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\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t200 Test<\/p>\n<\/td>\n

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\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t500 Test<\/p>\n<\/td>\n

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\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t1000 Test<\/p>\n<\/td>\n

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\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t2000 Test<\/p>\n<\/td>\n<\/tr>\n

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\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t2X One Step RT-PCR Mix<\/p>\n<\/td>\n

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\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t1×500 ul<\/p>\n<\/td>\n

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\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t1×1 ml<\/p>\n<\/td>\n

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\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t2×1 ml<\/p>\n<\/td>\n

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\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t5×1 ml<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t10×1 ml<\/p>\n<\/td>\n

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\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t20×1 ml<\/p>\n<\/td>\n<\/tr>\n

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\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t5X Primer Probe Mix<\/p>\n<\/td>\n

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\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t1×200 ul<\/p>\n<\/td>\n

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\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t1×400 ul<\/p>\n<\/td>\n

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\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t1×800 ul<\/p>\n<\/td>\n

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\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t2×1 ml<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t4×1 ml<\/p>\n<\/td>\n

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\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t8×1 ml<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n

<\/p>\n

\n\t\t\t\t\t\t\t\t\t\tStorage
\n\t\t\t\t\t\t\t\t\t\t<\/h5>\n

\n\t\t\t\t\t\t\t\t\t\tFor 20 pl total reaction volume;\n\t\t\t\t\t\t\t\t\t\t<\/p>\n<\/p>\n

\n\n\n\n\n\n\n
Content\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/td>\nAmount\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/td>\nFinal Concentration\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/td>\n<\/tr>\n
2X One Step RT-PCR Mix\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/td>\n10 ul\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/td>\n1X\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/td>\n<\/tr>\n
5X Primer Probe Mix\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/td>\n4 ul\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/td>\n1X\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/td>\n<\/tr>\n
Sample\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/td>\n6 ul\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n<\/p>\n

\t\t\t\t\t\t\t\t\t\t1-<\/b>The prepared RT-PCR sample is gently mixed and centrifuged briefly to collect
\nthe remaining drops on the walls of the tube completely to the bottom of the tube.<\/p>\n

\n\t\t\t\t\t\t\t\t\t\t2-<\/b>In PCR application, apply the following thermal cycle conditions.\n\t\t\t\t\t\t\t\t\t\t<\/p>\n<\/p>\n

\n\n\n\n\n\n\n\n\n
\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tNumber of Cycle<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tTemperature<\/p>\n<\/td>\n

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\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tDuration<\/p>\n<\/td>\n<\/tr>\n

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\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t1<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t55\u00b0C<\/p>\n<\/td>\n

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\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t15 Min<\/p>\n<\/td>\n<\/tr>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t1<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t95\u00b0C<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t1 Min<\/p>\n<\/td>\n<\/tr>\n

\n

\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t45\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t94\u00b0C<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t5 Sec<\/p>\n<\/td>\n<\/tr>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t60\u00b0C<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t20 Sec<\/p>\n<\/td>\n<\/tr>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tFAM\/HEX\/Cy5 reading<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n<\/p>\n

Evaluation of Results
\n\t\t\t\t\t\t\t\t\t\t<\/h5>\n<\/p>\n
\n\n\n\n\n\n\n\n\n\n
\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tFAM<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tHEX<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tCY5<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tInterpretation<\/p>\n<\/td>\n<\/tr>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t+<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t+<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t+<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tPositive<\/p>\n<\/td>\n<\/tr>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t–<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t–<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t+<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tNegative<\/p>\n<\/td>\n<\/tr>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t+<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t+<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t–<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tUnefficient RNA isolation \/ Positive<\/p>\n<\/td>\n<\/tr>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t–<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t+<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t+<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tPositive<\/p>\n<\/td>\n<\/tr>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t+<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t–<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t+<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tPositive<\/p>\n<\/td>\n<\/tr>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t–<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t–<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t–<\/p>\n<\/td>\n

\n

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tRepeat<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n<\/p>\n

\n\t\t\t\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t\t\t\t\"Covid-19\n\t\t\t\t\t\t\t\t\t\t<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/p><\/div>\n<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"

Covid-19 RT-PCR Detection Kit MD-COVID19-01 Description The HibriGen COVID-19 RT-PCR Kit is used for the qualitative detection of the SARS-CoV-2 (2019-nCoV) virus by RNA obtained […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"sablonlar-tr\/custom-post.php","meta":{"footnotes":""},"categories":[],"tags":[],"class_list":["post-54","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/hibrigen.com\/en\/wp-json\/wp\/v2\/pages\/54","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/hibrigen.com\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/hibrigen.com\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/hibrigen.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/hibrigen.com\/en\/wp-json\/wp\/v2\/comments?post=54"}],"version-history":[{"count":4,"href":"https:\/\/hibrigen.com\/en\/wp-json\/wp\/v2\/pages\/54\/revisions"}],"predecessor-version":[{"id":60,"href":"https:\/\/hibrigen.com\/en\/wp-json\/wp\/v2\/pages\/54\/revisions\/60"}],"wp:attachment":[{"href":"https:\/\/hibrigen.com\/en\/wp-json\/wp\/v2\/media?parent=54"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/hibrigen.com\/en\/wp-json\/wp\/v2\/categories?post=54"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/hibrigen.com\/en\/wp-json\/wp\/v2\/tags?post=54"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}