The 50 bp DNA ladder plus is ideal for determining double chain DNA sizes between 50 and 1000 base pairs. Ladder plus contains 13 linear double chain fragments of different sizes. The fragments with 250 and 500 base pairs were increased to allow easy identification. All fragments were measured precisely by both chromatography and HPLC method and mixed during production. For 5 µl loading, all fragments, except 250 bp and 500 bp, are 40 ng, and fragments with 250 bp and 500 bp are 100 ng. It is pre- mixed with Ladder loading paint and is ready for use.