HibriGen 2X TaqMan Master Mix is a pre-mixed ready-to-use solution; Taq DNA Polymerase contains reaction buffer in optimal concentration for efficient amplification of template DNA by dNTPs, Mg+2 and PCR. It is specially designed for TaqMan probe-based real-time PCR analysis of DNA samples (2X TaqMan Master Mix is designed for highly efficient quantitative PCR using TaqMan® probe- based chemistry). To prepare the PCR mixture, it is sufficient to add only template DNA and primary-probe. This pre-mixed formulation is ideal to save time and prevent contamination that may occur during the pipetting steps required for PCR setup. The mixture retains all the properties of Taq DNA Polymerase. Taq DNA Polymerase is suitable for amplification of target DNA up to 5 kb. Elongation rate is ~ 0.9-1.2kb / min (70-75°C). It has 5 ‘to 3’ polymerase activity.