HibriGen 2X B Master Mix is a premixed ready-to-use solution; Taq DNA Polymerase contains Reaction Buffer in optimal concentration for efficient amplification of template DNA by dNTPs, Mg+2 and PCR. To prepare the final PCR reaction, it is sufficient to add only template DNA and primers. This pre-mixed formulation is ideal to save time and prevent contamination that may occur during the pipetting steps required for PCR setup. The mixture retains all the properties of Taq DNA Polymerase. Taq DNA Polymerase is suitable for amplification of target DNA up to 5 kb. Elongation rate is ~ 0.9-1.2 kb/minute (70-75°C). It has 5‘- 3’ polymerase activity, 5‘- 3’ exonuclease activity; however, there is no 3‘- 5’ exonuclease activity.