Hot Start Taq DNA Polymerase
Concentration: 5U/µl Hot Start Taq DNA Polymerase is a special chemically modified Taq polymerase, the enzyme activity depends on the temperature increase and the enzyme is inactive at room temperature. In this way, it provides higher specificity by reducing non-specific products. The amplification length is up to 5 kb and the amplification speed can reach 2 min/kb (up to 20 s/kb). Hot Start Taq Polymerase has 5‘- 3’ polymerase activity, but 3‘- 5’ exonuclease activity. Creates PCR products containing 3‘ poly A tails that can be used in TA cloning. Since Hot Start Taq DNA Polymerase is created with advanced chemical modification, the use of animal resources is zero. It is much more stable than the antibody-modified Hot Start polymerase. Productivity; higher than chemically modified polymerase and the initial denaturation time can be reduced up to 3 minutes.
High specificity; It has the ability to reduce non-specific products as its chemical modification is active at high temperature
High Sensitivity; Capable of capturing target DNA with low copy number
Thermostable; More than 40 minutes half-life in 95°C incubation Accept modified nucleotides (eg Biotin, digoxigenin, etc.) as substrate Produce 3’-dA protruding PCR products
• Amplification of DNA fragments up to 5 kb
• High specificity routine PCR applications
• Colony PCR
• Genotyping
Hot Start Taq DNA Polymerase (5 U/μl)
50 µl Hot Start Buffer (Mg+2 plus) 1.25 ml