HibriGen 2X SYBR Green qPCR Mix (High Rox +) is designed for high efficiency and high performance Real Time PCR (qPCR). The kit contains Taq DNA Polymerase enzyme developed by molecular evolution process. As a result, it is a unique mixture prepared specifically for qPCR using SYBR Green I biochemistry. HibriGen 2X SYBR Green qPCR Mix (High Rox +) is a suitable premix of components (excluding primers, template DNA and water) for performing real-time Polymerase Chain Reaction (PCR) using SYBR Green I dye with improved precision and specificity. SYBR Green I dye binds to double chain DNA, thereby emitting fluorescent signal showing the amount of double chain DNA formed during PCR. This product is used for the detection and amplification of DNA during qPCR in the ABI real-time device, which provides normalization with a high Rox reference dye with a final concentration of 500 nM.