It is used to obtain genomic or non-genomic DNA or RNA using certain chemicals from human, animal, plant and microorganisms. The system is based on the disintegration of the cells, the unraveling of the DNA RNA and the attachment of this DNA RNA to the sephadex columns.

DNA isolation kits are standardized packages that enable us to isolate genomic and non-genomic DNA. While DNA isolation kits shorten the experimental stages, they enable us to perform DNA isolation more effectively. The kit we produce consists of platforms that allow DNA to bind (column). DNA isolation kit based on spin column method provides both time advantage and clarity in results.

While DNA and RNA, which are used in routine tests and in many different fields (disease determinations, laboratory applications, researches, genetic tests, genetic disease determinations, etc.) are routinely extracted in the laboratory, it is aimed to minimize the loss of time and changes in the results.

It is necessary to carefully process the DNA molecules to be isolated in large pieces. Different methods are required when isolating large DNA fragments, since large fragments tend to break and be damaged, rather than smaller fragments.

Agarose gel electrophoresis is a standard method used for the separation, identification and purification of DNA molecules. Technically; it is capable of resolving DNA fragments that are simple, fast and cannot be separated by other procedures. In addition, it is possible to determine the size of DNA in the gel by painting DNA with dyes that give fluorescent radiation at low concentrations.

Gel electrophoresis is a widely used molecular investigation method to determine the molecular weight, amount and subtypes of purified nucleic acid and proteins. Electrophoretic analysis is based on the principle that molecules dissolved in an electrical field migrate according to their electrical charges. The concentration of agarose gel varies depending on the size of the base pair of DNA.

In order to determine the location of the molecule used on the gel, it is necessary to have ethidium bromide (EB) or a similar brightening agent, which has a fluorescent effect under UV light. Today, SYBR Safe Gel Paint, which is not carcinogenic and has the same properties, is used instead of EtBr.

Electrophoresis experiments are carried out in buffer solutions. Buffers used in natural double-chain DNA electrophoresis usually contain EDTA and Tris-acetate (TAE) or Tris-borate (TBE).

After the gel and buffers are prepared, the DNA sample is loaded together with the 6X Loading dye, which increases the density of the sample, allowing the DNA to spread evenly into the well, adding the color to the well, facilitating the loading of the well and moving the sample towards the anode at a predictable speed in the electric field. is provided.

Finally, in order to determine the size of the DNA, “Ladder” called marker DNA of different sizes are loaded according to the need. We have ladders according to the desired length from the smallest size (20 bp) to the largest paint (1 kb plus).

As our company, we offer the agarose, SYBR Safe Gel Paint, TAE / TBE buffers, Loading Paint and Ladders mentioned here.