The 1 kb ladder (marker) is ideal for determining double chain DNA sizes between 500 and 10000 base pairs. Ladder contains 10 linear double chain fragments of different sizes. The fragments with 2000 and 5000 base pairs were increased to allow easy identification. All fragments were measured precisely by both chromatography and HPLC method and mixed during production. For 5 µl loading, all fragments, except 2000 bp and 5000 bp, are 40 ng, and fragments with 2000 bp and 5000 bp are 100 ng. It is pre-mixed with Ladder loading paint and is ready for use.