HibriGen 2X Hot Start Taq Master Mix is a pre-mixed ready-to-use solution; Hot Start Taq DNA Polymerase contains reaction buffer at optimal concentration for efficient amplification of template DNA by dNTPs, Mg+2 and PCR. In order to be ready for the PCR reaction, it is sufficient to add only template DNA and primers. This pre-mixed formulation is ideal to save time and prevent contamination that may occur during the pipetting steps required for PCR setup. The mixture preserves all the properties of Hot Start Taq DNA Polymerase. Hot Start Taq DNA Polymerase is suitable for amplification of target DNA up to 5 kb. Elongation rate is ~ 0.9-1.2 kb/minute (70-75°C). It has 5‘ to 3’ polymerase activity, but in the absence of 3‘ to 5’ exonuclease activity, 3’-dA protrusions (Poly-A tails) appear in the PCR product.