HibriGen 2X SYBR Green qPCR Mix (Low Rox +) is designed for high efficiency and high performance Real – Time PCR. The kit contains Taq DNA Polymerase enzyme developed by molecular evolution process. As a result, it is a unique mixture prepared specifically for qPCR using SYBR Green I biochemistry. HibriGen 2X SYBR Green qPCR Mix (Low Rox +) is a suitable premix of components (excluding primers, template DNA and water) for performing real-time Polymerase Chain Reaction (PCR) using SYBR Green I dye with improved precision and specificity. SYBR Green I dye binds to double chain DNA, thereby emitting fluorescent signal showing the amount of double chain DNA formed during PCR. This product is used for the detection and amplification of DNA during qPCR in the ABI real-time device, which provides normalization with Low Rox reference dye with a final concentration of 25 nM.
• This product can be used in ABI Real-time systems that require low concentration of Rox reference dye.
• Gene expression analysis
• DNA / RNA target detection
• Number of copies variation analysis