HibriGen 2X Taq Master Mix is a pre-mixed ready-to-use solution; Taq DNA Polymerase contains reaction buffer in optimal concentration for efficient amplification of template DNA by dNTPs, Mg+2 and PCR. In order to be ready for the PCR reaction, it is sufficient to add only template DNA and primers. This pre-mixed formulation is ideal to save time and prevent contamination that may occur during the pipetting steps required for PCR setup. The mixture retains all the properties of Taq DNA Polymerase. Taq DNA Polymerase is suitable for amplification of target DNA up to 5kb. Elongation rate is ~ 0.9-1.2kb / minute (70-750C). It has 5 ‘- 3’ polymerase activity, 5 ‘- 3’ exonuclease activity; however, there is no 3 ‘- 5’ exonuclease activity.
Handy; In order to be ready for PCR reaction, only primer and template DNA is added and it provides minimum optimization opportunity
High efficiency; Saves time by simplifying the process steps Repeatability; Minimizes the risk of errors caused by contamination and incorrect pipetting
• PCR with high product output
• Routine PCR with high repeatability
• PCR product formations for TA Cloning