TAE (Tris-Acetate-EDTA) is suitable for use in molecular biology at a concentration of 50X. Liquid form in 50X concentration can be diluted with distilled water or deionized water, making it easy to prepare 1X working solution. The pH (at 1X concentration) is in the range of 8.0-8.2 at 25°C. It does not contain protease, DNase and RNase. TAE buffer is generally used in all DNA electrophoresis (for acrylamide and agarose gel) applications including sequencing. TAE buffer is often used to ensure that fragments higher than 1500 bp are seen at better resolution.