6x Gel Loading Paint is a loading buffer mixed with two tracing dyes (Bromophenol blue and xylene cyanol) for use in DNA samples in (Blue) agarose and non-denatured polyacrylamide gel electrophoresis. It contains EDTA to chelate magnesium (up to 10mM) in the enzymatic reaction, so the reaction is stopped. Bromophenol blue and xylene cyanol are standard tracing paints for electrophoresis.
Two colors for tracking DNA progression during electrophoresis Gel does not mask DNA when exposed to UV light
EDTA binds to divalent metal ions and inhibits metal-dependent nucleases
Preparation of DNA ladder, marker and samples for loading agarose or polyacrylamide gel