6X Gel Loading Paint (Orange) is a loading buffer mixed with single monitoring paint for agarose and non-denatured polyacrylamide gel electrophoresis. It contains EDTA to chelate magnesium (up to 10mM) in the enzymatic reaction, so the reaction is stopped. Orange G is the standard tracking paint for electrophoresis.
Single color for tracking DNA progression during electrophoresis Gel does not mask DNA when exposed to UV light
EDTA binds to divalent metal ions and inhibits metal-dependent nucleases
Preparation of DNA ladder, marker and samples for loading onto agarose or polyacrylamide gel