Bacterial Genomic DNA Isolation Kit
HibriGen Bacteria Genomic DNA Isolation Kit; It performs genomic DNA isolation from Gram Negative and Gram Positive Bacteria with a fast and simple method without spinning phenol / chloroform, using spin column technology. No homogenization is required, tissue lysis (lysis) is carried out directly by proteinase-K. The buffer system has been optimized to selectively bind DNA to the column. Contaminants such as protein, divalent ions and secondary metabolites are easily removed with a simple centrifuge process. Pure DNA is dissolved in water or low-salt solution and is ready for use. DNA purified; free from contaminants and enzyme inhibitors, A260/A280 is between 1.7 and 1.9 and suitable for use in different applications.
High efficiency; 3-20 µg genomic DNA yield from 1.5-2 ml bacterial culture
Security; No phenol / chloroform step
Easy to use; Rapid purification with column filtration system, easily homogenizing with the help of proteinase-K without requiring mechanical disintegration
High purity; PCR, enzyme cutting etc. suitable for applications
DNA purified; free from contaminants and enzyme inhibitors; A260/A280 value is between 1.7 and 1.9; Suitable for use molecular biology applications such as:
• Enzyme cutting applications
• PCR
• Sanger and Next Generation Sequencing
• Labeling
• Library installation