The HibriGen Stool DNA Isolation Kit is a kit for microbial and host genomic DNA extraction from fresh or frozen feces, which performs DNA isolation simply and quickly using spin column technology without using phenol / chloroform. No homogenization is required, tissue lysis (lysis) is carried out directly by proteinase-K. The buffer system has been optimized to selectively bind DNA to the column. Contaminants such as protein, divalent ions and secondary metabolites are easily removed with a simple centrifuge process. Pure DNA is dissolved in water or low salt solution and is ready for use. DNA purified; free from contaminants and enzyme inhibitors, A260/A280 value is between 1.7 and 1.9 and suitable for use in different applications.
High efficiency; 3-35 μg genomic DNA yield
Security; No phenol / chloroform step
Easy to use; Rapid purification with the filter system, with the help of Proteinase-K, easy tissue disintegration without the need for mechanical disintegration
High Purity; PCR, enzyme cutting etc. suitable for applications
DNA purified; free from contaminants and enzyme inhibitors; A260/A280 value is between 1.7 and 1.9; Suitable for use in molecular biology applications such as:
• Enzyme cutting applications
• PCR
• Sanger and Next Generation Sequencing
• Labeling
• Library installation