The HibriGen Genomic DNA Isolation Kit spins DNA isolation from total blood (frozen up to 2 years and / or stored at 4°C) and mammalian tissues (fresh or frozen at -70 ° C until use) or cell culture without using phenol / chloroform. Performs simply and quickly with column technology. No homogenization is required, tissue lysis (lysis) is carried out directly by proteinase-K. The buffer system has been optimized to selectively bind DNA to the column. Contaminants such as protein, divalent ions and secondary metabolites are easily removed with a simple centrifuge process. Pure DNA is dissolved in water or low-salt solution and is ready for use. DNA purified; free from contaminants and enzyme inhibitors, A260/A280 is between 1.7 and 1.9 and suitable for use in different applications.
High efficiency; 3.5-30 μg genomic DNA yield
Security; No phenol / chloroform step
Easy to use; Rapid purification with filter system, easily tissue disintegration with the help of proteinase-K without requiring mechanical disintegration
High purity; PCR, enzyme cutting etc. suitable for applications
DNA purified; free from contaminants and enzyme inhibitors; A260 / A280 value is between 1.7 and 1.9; Suitable for use in molecular biology applications such as:
• Enzyme cutting applications
• PCR
• Sanger and Next Generation Sequencing
• Labeling
• Library installation