HibriGen Hibrizol is a ready-to-use reagent for total RNA isolation from tissues and cells. The reagent is a single-phase solution of phenol and guanidium isothiocyanate, which allows RNA isolation to be done in one step. During the homogenization or lysis stage, HibriGen Hibrzol protects the integrity of the RNA while ensuring the cell disruption and dissolution of the cell components. The solution is divided into two phases as organic and aqueous phase by centrifugation after chloroform addition. At this stage, RNA remained in the aqueous phase. After transferring the aqueous phase, RNA is recovered by precipitation with isopropyl alcohol. After removing the aqueous phase, the DNA and proteins in the sample are recovered by a second precipitation process. DNA is obtained from the intermediate phase using precipitation with ethanol. Proteins are recovered from the organic phase by additional precipitation with isopropyl alcohol. Auxiliary purification processes of DNA can be useful for normalizing RNA yield from sample to sample. This technique performs well in small amounts of tissue (50 – 100 mg) and cells (5 × 106) or large amounts of tissue (≥1 g) and cells (> 107) of human, animal, plant or bacterial origin. The simplicity of the Hibrizol method allows multiple samples to be processed simultaneously. The entire procedure can be completed within an hour. Total RNA isolated using Hibrizol does not contain DNA and protein contamination. For use in the isolated RNA Polymerase chain reaction (PCR), the isolated RNA is treated with DNase I suitable for amplification when two primers are contained in a single exon.