LongTaq DNA Polymerase
Concentration: 5U/µl LongTaq DNA polymerase was created by combining two thermostable DNA polymerases, Taq and Pfu DNA polymerase, with a special formulation to amplify large fragments. This specially formulated LongTaq DNA Polymerase has been shown to amplify long patterns such as λ phage genomes up to 20 kb. LongTaq DNA Polymerase is also a good choice for amplifying complex pattern DNA such as GC rich regions. Using LongTaq DNA Polymerase in the PCR reaction creates PCR products containing 3‘ poly A tails that can be used in TA cloning.
High accuracy; 3 times more accuracy than Taq polymerase Longer fragments; amplify long-form DNA up to 20 kb Creates 3 ‘Poly A tails and blunt end PCR products
• PCR amplification of complex pattern DNA (eg GC rich patterns and repeating sequences)
• PCR amplification of long form DNA
• DNA sequencing
• PCR for cloning
LongTaq DNA Polymerase 50 μl
10xPCR Buffer I (KCl) 1.25 ml
10xPCR Buffer II (KCl ve NH2SO4 ) 1.25 ml
PCR Booster (Enhancer) 500 μl