The HibriGen PCR and DNA Fragment Purification Kit is designed to quickly and effectively purify mixtures from PCR products, DNA fragments and other enzymatic reactions. The kit simply and quickly performs with spin column technology without the use of demanding resin processes or phenol / chloroform. The HibriGen PCR and DNA Fragment Purification Kit effectively removes salt, enzymes, indeterminate nucleotides, dNTPs and primers from the PCR and other reaction mixtures. The kit can be used to purify DNA fragments in the range of 50 bp to 10 kb and can provide up to 90% recovery. Each purification column has a total DNA binding capacity of up to 45μg and the whole procedure takes only 7 minutes. DNA purified; It is suitable for use in cloning, labeling, ligation, blotting, in situ hybridization or in vitro transcription applications.
Speed; Quick procedure completed in 7 minutes
High efficiency; Up to 90% recovery of DNA fragments in the range of 50 bp – 10 kb
Practical; Capped filters combined with collecting tubes
High purity; OD260 / 280 = 1.7-1.9 with high purity ready for use in later applications such as DNA, PCR and restriction cutting
• Conventional restriction cutting
• Automatic fluorescent or radioactive sequencing
• PCR
• in vitro transcription