Concentration: 5U/µl Pfu DNA Polymerase is a recombinant DNA Polymerase enzyme, which has a much higher proofreading property and thermal stability than other DNA polymerases obtained from Pyrococcus furiosus organism, which is a hyperthermophilic archaea and has a molecular weight of 90 kDa. It is suitable for amplification of target DNA up to 2 kb. The elongation rate is ~ 0.2 – 0.4 kb/minute (70-75°C). Pfu DNA Polymerase has a 3’ – 5‘ exonuclease proofreading activity, which allows for the correction of incorrect binding of nucleotides. Thanks to this feature, PCR products made using Pfu DNA polymerase have less error than PCR products made using Taq DNA polymerase. Using Pfu DNA Polymerase, blind-end PCR products suitable for use in blind-end vectors used in cloning can be obtained. Pfu DNA Polymerase is the best technique required for high-throughput DNA synthesis.