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NUCLEIC ACID EXTRACTION AND ANALYSIS

Standart PCR ve QPCR Reactives

  • Blood Genomic DNA Isolation Kit performs DNA isolation from total blood (frozen up to 2 years and / or stored at 4 ° C) with silica-gel-membrane technology in a simple and rapid way without using phenol / chloroform. Homogenization is not required, the fragmentation (lysis) of the tissue is directly effected by the proteinase-K.

    The buffer system is optimized to allow DNA to selectively bind to the silica-gel-membrane. By simple centrifugation, contaminants such as protein, divalent ions and secondary metabolites are readily removed.

    Pure DNA is dissolved in water or low-salt solution and ready for use. Purified DNA; free from contaminants and enzyme inhibitors; The A260 / A280 is between 1.7 and 1.9 and is suitable for different downstream applications.

    • Features High Quality; 3-10 µg genomic DNA yield in all sample types.
    • High Reliability; Lack of phenol / chloroform step.
    • User-friendly; Quick purification with filter system, tissue breakdown with the help of proteinase-K
    • High Purity; Suitable for a wide range of downstream applications such as PCR, enzyme cleavage etc.
    • Applications
    • • Sequencing
    • • Enzyme cleavage
    • • PCR
    • • Southern Blotting
    • Cat No:
    • 50 Preps : MG-KDNA-02-50
    • 100 Preps : MG-KDNA-02-100
    • 250 Preps : MG-KDNA-02-250
  • Tissue/Culture Cells Genomic DNA Purification Kit provides a simple and rapid method for high quality genomic DNA purification from mammalian tissues(either fresh or frozen at -70°C until use) and culture cells. The Tissue/Culture Cells Genomic DNA system uses the silica-gel-membrane technology for simple and fast isolation of Genomic DNA without phenol/chloroform. Homogenization is not necessary since tissues are directly lysed by Proteinase K.

    The buffer system is optimized to allow selective binding of DNA to the silica-gel membrane. The simple centrifugation protocol completely removes contaminants such as proteins, divalent cations, and secondary metabolites. Pure DNA is then eluted in water or low-salt buffer, ready to use.

    The typical yield of genomic DNA is 3-35 μg from 10 mg of tissue or 1 x 106-107 culture cells. The purified high molecular weight genomic DNA is suitable for direct use in all common molecular biology applications: PCR, restriction digestion, cloning, DNA sequencing and Southern Blot analysis.

    • Features High Quality; 3-35 µg genomic DNA yield in all sample types.
    • Safety; Lack of phenol / chloroform step.
    • User-friendly; Quick purification with filter system, tissue breakdown with the help of proteinase-K
    • High Purity; Suitable for a wide range of downstream applications such as PCR, enzyme cleavage etc.
    • Applications
    • • Restriction digestion
    • • Labeling
    • • PCR
    • • Library construction
    • Cat No:
    • 50 Preps : MG-QTDNA-01-50
    • 100 Preps : MG-QTDNA-01-100
    • 250 Preps : MG-QTDNA-01-250
  • HibriGen Ancient DNA Extraction From Bones and Teeth Kit provides a simple and fast method for isolation of high quality DNA from bone or tooth tissue. The kit provides a rapid and simple isolation of genomic DNA using silica gel membrane technology without using phenol / chloroform.

    Homogenization is not necessary since the tissues are directly degraded by the proteinase K. Kit solutions are specifically designed and optimized for the selective binding of the DNA to the silica gel membrane. By simple centrifugation, contaminants such as protein, divalent cations and secondary metabolites are completely removed. Then pure DNA is made ready for use with pure water or low salt solution.

    Generally, 2-5 μg genomic DNA yield is obtained from 10 mg tissue. The purified high molecular weight genomic DNA is ideal for use in molecular biology applications such as enzyme cleavage, PCR, labeling, library construction.

    • Features High Quality; 2-5 µg genomic DNA yield in all sample types.
    • Safety; Lack of phenol / chloroform step.
    • User-friendly; Quick purification with filter system, tissue breakdown with the help of proteinase-K
    • High Purity; Suitable.
    • Applications
    • • Enzyme cleavage
    • • Labeling
    • • PCR
    • • Library constraction reactions
    • Cat No:
    • 100 Preps: MG-ANCDNA-01-100
  • In progress...

    • Cat No:
    • 50 Preps: MG-TDNA-01-50
    • 100 Preps: MG-TDNA-01-100
    • 250 Preps: MG-TDNA-01-250
  • The Bacteria Genomic DNA Extraction Kit provides a simple and rapid method for high quality genomic DNA purification from Gram Positive and Gram Negative Bacteria. The Bacteria Genomic DNA system uses the silica-gel-membrane technology for simple and fast isolation of Genomic DNA without phenol/chloroform.

    Homogenization is not necessary since tissues are directly lysed by Proteinase K. The buffer system is optimized to allow selective binding of DNA to the silica-gel membrane. The simple centrifugation protocol completely removes contaminants such as proteins, divalent cations, and secondary metabolites.

    Pure DNA is then eluted in water or low-salt buffer, ready to use. The typical yield of genomic DNA is 3-20 µg from 0.5–2 ml of bacteria culure. The purified high molecular weight genomic DNA is suitable for direct use in all common molecular biology applications.

    • Features Efficient;3-20 µg of genomic DNA from 1.5–2 ml bacteria culture.
    • Fast; Procedure takes only 30 min.
    • Safe;No phenol extraction step.
    • High purity; Purified DNA is ready for downstream application such as PCR, restriction digestion.
    • Applications
    • • Sequencing
    • • Labeling
    • • PCR
    • • Library construction
    • Cat No:
    • 50 Preps : MG-BDNA-01-50
    • 100 Preps: MG-BDNA-01-100
    • 250 Preps: MG-BDNA-01-250
  • The Yeast Genomic DNA Extraction Kit provides a simple and rapid method for high quality genomic DNA purification from yeast. The Yeast Genomic DNA system uses the silica-gel-membrane technology for simple and fast isolation of Genomic DNA without phenol/chloroform. Homogenization is not necessary since tissues are directly lysed by Proteinase K.

    The buffer system is optimized to allow selective binding of DNA to the silica-gel membrane. The simple centrifugation protocol completely removes contaminants such as proteins, divalent cations, and secondary metabolites. Pure DNA is then eluted in water or low-salt buffer, ready to use.

    The typical yield of genomic DNA is 3-25 µg from 1–5 x 107 yeast cells. The purified high molecular weight genomic DNA is suitable for direct use in all common molecular biology applications: PCR, restriction digestion, cloning, DNA sequencing and Southern blot analysis.

    • Features High Quality; 3-25 µg genomic DNA yield in all sample types.
    • Safety; Lack of phenol / chloroform step.
    • User-friendly; Quick purification with filter system, tissue breakdown with the help of proteinase-K
    • High Purity; Suitable for a wide range of downstream applications such as PCR, enzyme cleavage etc.
    • Applications
    • • Restriction digestion
    • • Labeling
    • • PCR
    • • Library construction
    • Cat No:
    • 50 Preps: MG-YDNA-01-50
    • 100 Preps: MG-YDNA-01-100
    • 250 Preps: MG-YDNA-01-250
  • In progress...

    • Features
    • Applications
    • Cat No:
    • 50 Preps: MG-BPDNA-01-50
    • 100 Preps: MG-BPDNA-01-100
    • 250 Preps: MG-BPDNA-01-250
  • This total RNA extraction kit provides a simple method of isolating total RNA from wide range of sample types (tissue, adherent or suspension cells, total blood) and amounts. Samples are lysed and then homogenized in the presence of guanidinium isothiocyanate, a chaotropic salt capable of protecting RNA from endogenous RNases.

    After homogenization, ethanol is added to the sample. The samples are then processed through a spin column containing a clear silica-based membrane to which the RNA binds. Any impurities are effectively removed by subsequent washing step.

    The purified RNA is then eluted in RNase-free water and is suitable for use in a variety of downstream applications.

    • Features High Quality; 1-3 µg RNA yield from all sample types.
    • Safety; Lack of phenol / chloroform step.
    • User-friendly; Quick purification with filter system, tissue breakdown the lack of proteinase-K
    • High Purity; Suitable for a wide range of downstream applications such as QRT-PCR, Northern Blotting,cDNA library construction etc.
    • Applications
    • • Real-time PCR (RT-PCR)
    • • Northern Blot
    • • Nuclease protection assays
    • • RNA amplification for microarray analysis
    • • cDNA library preparation
    • Cat No: MG-RNA-01
    • 50 Preps: MG-RNA-01-50
    • 100 Preps: MG-RNA-01-100
  • Trizol Reagent is a ready-to-use reagent for the isolation of total RNA from cells and tissues. The reagent, a mono-phasic solution of phenol and guanidine isothiocyanate, is an improvement to the single-step RNA isolation method. During sample homogenization or lysis, Trizol Reagent maintains the integrity of the RNA, while disrupting cells and dissolving cell components.

    Addition of chloroform followed by centrifugation, separates the solution into an aqueous phase and an organic phase. RNA remains exclusively in the aqueous phase. After transfer of the aqueous phase, the RNA is recovered by precipitation with isopropyl alcohol. After removal of the aqueous phase, the DNA and proteins in the sample can be recovered by sequential precipitation. Precipitation with ethanol yields DNA from the interphase, and an additional precipitation with isopropyl alcohol yields proteins from the organic phase.

    Copurification of the DNA may be useful for normalizing RNA yields from sample to sample. This technique performs well with small quantities of tissue (50-100 mg) and cells (5 × 106), and large quantities of tissue (≥1 g) and cells (>107), of human, animal, plant, or bacterial origin. The simplicity of the Trizol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour. Total RNA isolated by Trizol Reagent is free of protein and DNA contamination.

    • Applications
    • RNA Northern Blotting
    • • Dot blot hibridisation
    • • Selection of Poly (A)+
    • • In vitro translation
    • • Rnaz Protection Assay
    • • Molecular cloning
    • Cat No:
    • 100 mL : MG-TRZ-01-100
    • 250 mL : MG-TRZ-01-250
    • 500 mL : MG-TRZ-01-500
  • Gel Extraction Kit is designed to extract and purify DNA fragments of 50bp to 40kb from standard or low-melt agarose gels in either Tris acetate (TAE) or Tris borate (TBE).

    This membrane-based system, which can bind up to 40μg DNA, allows recovery of isolated DNA fragments in as little as 25 minutes, depending on the number of samples processed and the protocol used.

    The purified DNA can be used for automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion or in vitro transcription/translation without further manipulation.

    • Features Fast; Procedure takes only 25 min.
    • High Efficient; up to 85% recoveries in the range of 50bp-40kb.
    • High purity ; OD260/280=1.7-1.9. Purified DNA is ready for downstream application such as PCR, restriction digestion.
    • 100 Reaction
    • Applications
    • • Restriction digestion
    • • DNA Sequencing
    • • PCR
    • • in vitro transcription
    • Cat No:
    • 50 Preps: MG-GEK-01-50
    • 100 Preps: MG-GEK-01-100
    • 250 Preps: MG-GEK-01-250
  • PCR and DNA Fragment Purification Kit is designed for rapid and efficient purification of PCR products, DNA fragments and other enzymatic reaction mixtures such as cycle sequencing. The kit uses silica-gel-membrane technology, which eliminates the need for difficult resin treatments or toxic phenol / chloroform extraction.

    HibriGen PCR and DNA Fragment Purification Kit effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes and salts from PCR and other reaction mixtures. The kit can be used for purification of DNA fragments from 50 bp to 40 kb with recovery rates up to 90 %.

    Each purification column has a total binding capacity of up to 30μg of DNA and the entire procedure takes just 7 minutes. The purified DNA can be used in common downstream applications such as sequencing, restriction digestion, labeling, ligation, cloning, in vitro transcription, blotting or in situ hybridization.

    • Features • Fast; Procedure takes only 7 minutes.
    • High Efficient ; up to 90 % recoveries in the range of 50bp-40kb.
    • Convenient;Filtreler kapaklıdır ve toplama tüpleri ile birleştirilmiştir.
    • High purity; OD 260/280 = 1.8-1.9. Purified DNA is ready for downstream application such as PCR, restriction digestion, sequencing.
    • 100 Reaction
    • Applications
    • • Sequencing
    • • Restriction digestion
    • • PCR
    • • Southern Hybridization
    • Cat No:
    • 50 Preps: MG-PDNA-01-50
    • 100 Preps: MG-PDNA-01-100
    • 250 Preps: MG-PDNA-01-250
  • SYBR SAFE is a new nucleic acid stain, and alternative to the traditional ethidium bromide (EB) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima. When bound to nucleic acid, one centered at 267 nm and another at 294 nm. In addition, it has one visible excitation at 491nm. The Fluorescence emission of GoldViewTM bound to DNA is centered at 530 nm.

    • Features Reliable; Less harmful than Ethidium Bromide.
    • High precision; Very high precision staining to display DNA on agarose or acrylamide gel.
    • Practical; Ready to use, blue light or UV radiation to receive images, Be able to bind RNA as well as DNA.
    • 1 mL
    • Applications
    • • 1. Sensitivity detection of SYBR SAFE under UV transmission (wave length 300nm)
    • • 2.Sensitivity detection of EB under UV transmission (wave length 300nm)
    • Cat No:
    • 1 mL: MG-SSGD-01-1000
    • 400 μl: MG-SSGD-01-400
  • 6x Gel Loading Dye is a loading buffer mixed with two tracking dyes (Bromophenol Blue and Xylene Cyanol) to be used for DNA samples in agarose and non-denatured polyacrylamide gel electrophoresis. It contains EDTA for chelating magnesium to stop the enzymatic reaction. Bromophenol Blue and xylene cyanol are the standard tracking dyes for electrophoresis.

    • Features Two-color tracking of DNA migration during electrophoresis
    • No DNA masking during gel exposure to UV light
    • EDTA binds divalent metal ions and inhibits metal dependent nucleases.
    • 1 mL
    • Application
    • Appropriate for analysis of DNA molecules, preparation of DNA ladders, markers and samples for loading on agarose or polyacrylamide gels.
    • Cat No:
    • 1 mL: MG-LDYB-01
  • 6x Gel Loading Dye is a loading buffer mixed with two tracking dyes (Orange G and xylene cyanol) to be used for DNA samples in agarose and non-denatured polyacrylamide gel electrophoresis. It contains EDTA for chelating magnesium to stop the enzymatic reaction. Orange G and xylene cyanol are the standard tracking dyes for electrophoresis.

    • Features Two-color tracking of DNA migration during electrophoresis
    • No DNA masking during gel exposure to UV light
    • EDTA binds divalent metal ions and inhibits metal dependent nucleases.
    • 1 mL
    • Application
    • Appropriate for analysis of DNA molecules, preparation of DNA ladders, markers and samples for loading on agarose or polyacrylamide gels.
    • Cat No:
    • 1 mL: MG-LDYO-01
  • In Progress ...

    • Features
    • 1 mL
    • Cat No: MG-LDYDUO-01
  • In Progress ...

    • Features
    • Application
    • Cat No:
    • 50μg: MG-LDR-20
  • 50bp ladder is ideal for determining the size of double-stranded DNA from 50 to 500 base pairs. The ladder consists of 8 linear double-stranded fragments of different lengths. The 250bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 μl loading, concentration of all fragments except 250bp are 40ng. The 250bp fragment is at 100ng concentration. This ladder is pre-mixed with loading dye and is ready to use.

    • Features 50 µg
    • Concentration: 76ng/µl
    • Bant concentration:
    • Typical Bands : 100 ng/5µl
    • Other Bands : 40 ng/5µl
    • Cat No:
    • 50μg: MG-LDR-50
  • 50bp ladder plus is ideal for determining the size of double-stranded DNA from 50 to 1000 base pairs. The ladder consists of 13 linear double-stranded fragments of different lengths. The 250bp and 500bp fragments are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 μl loading, concentration of all fragments except 250bp and 500bp are at 40ng. The 250bp and 500bp fragment are at 100ng concentration. This ladder is pre-mixed with loading dye and is ready to use.

    • Features 50 µg
    • Concentration: 136ng/µl
    • Band Concentrations:
    • Typical Bands: 100 ng/5µl
    • Other Bands : 40 ng/5µl
    • Cat No:
    • 50μg: MG-LDR-50P
  • 100bp ladder is ideal for determining the size of double-strand DNA from 100-1500 base pairs. The ladder consist of 11 linear double-strand fragments of different lengths. The 500bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5µl loading, concentration of all fragments except 500bp are 40ng. 500bp fragment is at 100ng cocnetration. The ladder is pre-mixed with loading dye and is ready to use.

    • Features 50 µg
    • Concentration: 136ng/µl
    • Band Concentrations:
    • Typical Bands: 100 ng/5µl
    • Other Bands: 40 ng/5µl
    • Cat No:
    • 50μg: MG-LDR-100
  • 100bp ladder plus is ideal for determining the size of double-stranded DNA from 100 to 3,000 base pairs. The ladder consists of 14 linear double-stranded fragments of different lengths. The 500bp and 1,200bp fragments are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 μl loading, concentration of all fragments except 500bp and 1,200bp are 40ng. The 500bp and 1,200bp fragments are at 100ng concentration. This ladder is pre-mixed with loading dye and is ready to use.

    • Features 50 µg
    • Concentration: 136ng/µl
    • Band Concentrations:
    • Typical Bands : 100 ng/5µl
    • Other Bands : 40 ng/5µl
    • Cat No:
    • 50μg: MG-LDR-100P
  • 1kb ladder is ideal for determining the size of double-stranded DNA from 500 to 10,000 base pairs. The ladder consists of 10 linear double-stranded fragments of different lengths. The 2,000bp and 5,000bp fragments are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 ul loading, concentrations of all fragments except 2,000bp and 5,000bp are 40ng. The 2,000bp and 5,000bp fragments are at 100ng concentration. This ladder is pre-mixed with loading dye and is ready to use.

    • Features 50 µg
    • Concentration: 104ng/µl
    • Band Concentrations:
    • Typical Bands : 100 ng/5µl
    • Other Bands : 40 ng/5µl
    • Cat No:
    • 50μg: MG-LDR-1000
  • 1 kb ladder plus is ideal for determining the size of double-stranded DNA from 100 to 10,000 base pairs. The ladder consists of 15 linear double-stranded fragments of different lenghts .The 500bp and 3,000bp fragments are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 μl loading, concentration of all fragments except 500bp and 3,000bp are 40ng. The 500bp and 3,000bp fragment are at 100ng concentration. This ladder is pre-mixed with loading dye and is ready to use.

    • Features: 50 µg
    • Concentration: 144ng/µl
    • Band Concentrations:
    • Typical Bands: 100 ng/5µl
    • Other Bands: 40 ng/5µl
    • Cat No:
    • 50μg: MG-LDR-1000P
  • The Hind III digest of lambda DNA yields 8 fragments of different size suitable for use as molecular weight standards for agarose gel electrophoresis. λDNA /Hind Ⅲ is pre-mixed with loading buffer and is ready to use.

    • Cat No:
    • 50μg: MG-LDR-LH3
  • TAE (Tris-Acetate-EDTA) is suitable for use in molecular biology at a concentration of 50X. At a concentration of 50X, the liquid form can be easily diluted with distilled water or deionized water to allow the preparation of working solution. The pH is in the range of 8.2-8.4 at 25 ° C (1X concentration). It does not contain protease, DNase and RNase.

    TAE buffer is generally used for all DNA electrophoresis (for acrylamide and agarose gel), including sequencing. TAE buffer is generally used to visualise fragments larger than 1500 bp in high resolution. Due to its low buffering capacity compared to TBE, TAE buffer can easily become exhausted.

    • Features: 500 mL
    • Cat No:
    • 500 mL: MG-TAE-500
    • 1000 mL: MG-TAE-1000
  • TBE (Tris-Borate-EDTA) is suitable for use in molecular biology at a concentration of 10X. At a concentration of 10X, the liquid form can be easily diluted with distilled water or deionized water to allow the preparation of 0.5X working solution. The pH is in the range of 8.2-8.4 at 25 ° C (1X concentration). It does not contain protease, DNase and RNase.

    TBE buffer is generally used for all DNA electrophoresis (for acrylamide and agarose gel), including sequencing. TBE buffer is generally used to visualise fragments smaller than 1500 bp in high resolution. Due to its high buffering capacity and low conductivity compared to TAE, TBE buffer is more suitable for electrophoresis application at high voltages (> 150V).

    • Features: Practical;Easy to use with distilled or iodized water.
    • High purity; Protease, DNase and RNase free, suitable for use in molecular biology.
    • High discrimination power;Ideal for working at high voltages, allowing smaller fragments to be observed on the gel at higher resolution.
    • 500 mL
    • Cat No:
    • 500 mL: MG-TBE-500
    • 1000 mL: MG-TBE-1000

  • 2X Taq master mix is a pre-mixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, Mg+2 and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To make it ready for the PCR reaction, it is sufficient to add only template DNA and primers. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. The mix retains all features of Taq DNA Polymerase.

    Taq DNA polymerase can amplify DNA target up to 5kb. The elognation velocity is ~0.9-1.2kb/min (70-750C). It has 5’ to 3’ polymerase activity but lacks of 3’ to 5’ exonuclease activity that results in 3’-dA overhangs PCR product.

    • Features: Convenient;only primers and template DNA are added when prepare final PCR.
    • High Yields ; of PCR products with minimal optimization.
    • High Efficiency;saving your time by simplifying the process.
    • Reproducible;lower contamination and pipetting error risk.
    • With dye;ready to agarose applications.
    • 500 mL
    • Application
    • • High throughput PCR
    • • Routine PCR with high reproducibility
    • • Generation of PCR products for TA cloning
    • Cat No:
    • 25 µl’lik 80 Reaks.: MG-TAQMX-01-80
    • 25 µl’lik 400 Reaks.: MG-TAQMX-01-400
  • 2X Taq master mix is a pre-mixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, Mg+2 and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To make it ready for the PCR reaction, it is sufficient to add only template DNA and primers. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. The mix retains all features of Taq DNA Polymerase.

    Taq DNA polymerase can amplify DNA target up to 5kb. The elognation velocity is ~0.9-1.2kb/min (70-750C). It has 5’ to 3’ polymerase activity but lacks of 3’ to 5’ exonuclease activity that results in 3’-dA overhangs PCR product.

    • Features: Convenient;only primers and template DNA are added when prepare final PCR.
    • High Yields ; of PCR products with minimal optimization.
    • High Efficiency;saving your time by simplifying the process.
    • Reproducible;lower contamination and pipetting error risk.
    • 500 mL
    • Application
    • • High throughput PCR
    • • Routine PCR with high reproducibility
    • • Generation of PCR products for TA cloning
    • Cat No:
    • 25 µl’lik 80 Reaks.: MG-TAQMX-02-80
    • 25 µl’lik 400 Reaks.: MG-TAQMX-02-400
  • Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 0.9~1.2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product.

    • Kit Contents 500U
    • Taq DNA Polymerase 100μl
    • 10xPCR Buffer KCl 1ml
    • 10xPCR Tampon NH2SO4 1ml
    • 25 mM MgCl2 1ml
    • 500 U
    • Applications
    • • DNA labeling
    • • DNA sequencing.
    • • PCR for cloning.
    • Cat No: MG-KTAQP-01
  • In progress...

    • Features
    • 250 U
    • Cat No: MG-LTAQP-01
  • In progress...

    • Features
    • 250 U
    • Cat No: MG-PFU-01
  • dNTP mix is an aqueous solution at ph 7.0 containing dATP, dGTP, dCTP, dTTp, each at final concentration of 10mM. This mix is designed to save time and to provide higher reproducibility in PCR and other applications. The mix offers possibility to reduce the number of pipetting steps and the risk of reaction set up errors...

      • Applications
      • For direct use in PCR,long PCR, RT-PCR, cDNA synthesis,primer extension, DNA sequencing and labeling.
      • Features
      • 1 mL
      • Cat No: MG-dNTP-025
  • The dNTP set contains 100 mM dATP, dCTP, dTTP, dGTP solutions, each prepared in a separate vial. By providing nucleotides separately, the dNTP Set offers maximum flexibility in the preparation of reaction mixtures for different applications.

    • Applications
    • For use directly in PCR, long PCR, RT-PCR, cDNA synthesis, primer extension, DNA sequencing and marking studies.
    • Volume:
    • 0.25ml 100mM
    • dATP 0.25ml
    • 100mM dCTP 0.25ml
    • 100mM dGTP 0.25ml
    • 100mM dTTP
    • Cat No: MG-dNTP-01
  • dNTP mix is an aqueous solution at ph 7.0 containing dATP, dGTP, dCTP, dTTp, each at final concentration of 10mM. This mix is designed to save time and to provide higher reproducibility in PCR and other applications. The mix offers possibility to reduce the number of pipetting steps and the risk of reaction set up errors.

    • Applications
    • For direct use in PCR,long PCR, RT-PCR, cDNA synthesis,primer extension, DNA sequencing and labeling.
      • Features
      • 1ml
      • Cat No: MG-dNTP-10
  • 2x SYBR® Green qPCR Mix is a convenient premix of the components (except primers, template, and water) necessary to perform real-time polymerase chain reaction (PCR) using SYBR® Green I dye with enhanced sensitivity and specificity. The SYBR® Green dye binds to double-stranded (ds) DNA, thus providing a fluorescent signal that reflects the amount of dsDNA product generated during PCR.

    • Features • This reagent can be used in glass capillary systems (LightCycler, Roche Molecular Systems,Inc. Etc.).
    • • This reagent can be used in a passive reference system. (ABI PRISM® 7700, Applied Biosystems, Inc. Etc.). The passive reference dye does not effect any other systems.
    • Applications
    • • Intercalation test with SYBR Green I • Real Time PCR • Real Time PCR -RT PCR
    • Cat No:
    • 25 µl’lik 80 Reaks.: MG-SYBR-01-80
    • 25 µl’lik 400 Reaks.: MG-SYBR-01-400
  • 2x SYBR® Green qPCR Mix (Low Rox+) is designed for high-performance, high-throughput, real-time PCR. The kit contains a Taq DNA polymerase engineeredthrough a process of molecular evolution. The result is a unique enzyme, specifically designed for qPCR using SYBR® Green I dye chemistry.

    2x SYBR® Green qPCR Mix (Low Rox+) is a convenient premix of the components (except primers, template, and water) necessary to perform real-timepolymerase chain reaction (PCR) using SYBR® Green I dye with enhanced sensitivity and specificity. The SYBR® Green dye binds to double-stranded (ds) DNA,thus providing a fluorescent signal that reflects the amount of dsDNA product generated during PCR. This reagent is used in amplification and detection of DNA in qPCR on ABI real-time instruments that support normalization with Rox reference dye at a final concentration of 50 nM.

    • Features • This reagent can be used in ABI Real-time systems that require low concentration of Rox reference dye.
    • Applications
    • • Gene expression analysis
    • • Low-copy number gen detection
    • • Microarray validation
    • • Gene knockdown validation
    • Cat No:
    • 25 µl’lik 80 Reaks.: MG-SYBR-LROX-01-80
    • 25 µl’lik 400 Reaks.: MG-SYBR-LROX-01-400
  • 2x SYBR® Green qPCR Mix (High Rox+) is designed for high-performance, high-throughput, real-time PCR. The kit contains a Taq DNA polymerase engineered through a process of molecular evolution. The result is a unique enzyme, specifically designed for qPCR using SYBR® Green I dye chemistry.

    2x SYBR® Green qPCR Mix (High Rox+) is a convenient premix of the components (except primers, template, and water) necessary to perform real-time polymerase chain reaction (PCR) using SYBR® Green I dye with enhanced sensitivity and specificity. The SYBR® Green dye binds to double-stranded (ds) DNA, thus providing a fluorescent signal that reflects the amount of dsDNA product generated during PCR. This reagent is used in amplification and detection of DNA in qPCR on ABI real-time instruments that support normalization with high Rox reference dye at a final concentration of 500 nM.

    • Features • This product can be used in ABI Real-time systems that require a high concentration of Rox reference dye.
    • Applications
    • • Gene expression analysis
    • • Low-copy number gen detection
    • • Microarray validation
    • • Gene knockdown validation
    • Cat No:
    • 25 µl’lik 80 Reaks.: MG-SYBR-HROX-01-80
    • 25 µl’lik 400 Reaks.: MG-SYBR-HROX-01-400
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