DNaz I, (RNaz içermeyen), di-, tri- ve salınımları serbest bırakmak üzere DNA’yı spesifik olmayan bir şekilde parçalayan bir endonükleazdır. 5’-fosforile edilmiş ve 3’-hidroksile uçlara sahip oligonükleotit ürünleri. DNaz I, tek ve çift sarmallı DNA, kromatin ve RNA: DNA melezleri.
Rekombinant enzim
• Transkripsiyon reaksiyonlarında DNA şablonunun bozulması
• Kirletici genomik DNA’nın RNA örneklerinden çıkarılması
• DNaz I footprinting (in vitro protein-DNA bağlanması analizi)
• Nick (tek iplik içeren) Translasyon
Sığır pankreasından izole edilmiştir.
38 kDa monomer
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