Konsantrasyonu: 5U/µl Taq DNA Polimeraz termofilik bir bakteri olan Thermus aquaticus bakterisinden elde edilen bir rekombinant DNA Polimeraz enzimidir. Moleküler ağırlığı 94 kDa olup, Taq DNA Polimeraz 5 kb’a varan hedef DNA’ların amplifikasyonuna uygundur. Uzama hızı ~0.9-1.2kb/ dakika (70-75°C)’dır. 5’ – 3’ polimeraz aktivitesine, 5’ – 3’ ekzonükleaz aktivitesine sahiptir. Ancak 3’ – 5’ ekzonükleaz aktivitesi yoktur.
• 5 kb’ a kadar uzunluktaki DNA fragmanlarının amplifikasyonu
• DNA işaretleme
• DNA dizileme
• Klonlama için PCR
Taq DNA Polimeraz 100 μl
10xPCR Tampon KCl 1.25 ml
10xPCR Tampon NH2SO4 1.25 ml
25 mM MgCl2 1.25 ml
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